MOLECULAR-CLONING OF BAC7, A PROLINE-RICH AND ARGININE-RICH ANTIMICROBIAL PEPTIDE FROM BOVINE NEUTROPHILS

被引：42

作者：

SCOCCHI, M

ROMEO, D

ZANETTI, M

机构：

[1] LAB NAZL CIB, I-34012 TRIESTE, ITALY

[2] UNIV TRIESTE, DIPARTIMENTO BIOCHIM BIOFIS & CHIM MACROMOLEC, I-34127 TRIESTE, ITALY

[3] UNIV UDINE, DIPARTIMENTO SCI & TECNOL BIOMED, I-33100 UDINE, ITALY

来源：

FEBS LETTERS | 1994年 / 352卷 / 02期

关键词：

CDNA; ANTIBACTERIAL PEPTIDE; BACTENECIN; NEUTROPHIL;

D O I：

10.1016/0014-5793(94)00954-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bac7 is a 7 kDa proline- and arginine-rich antimicrobial peptide which was purified from bovine neutrophils. We have used PCR to clone the cDNA of Bac7 precursor, a polypeptide of 21,569 Da. This cDNA is highly conserved in the 5' region, with respect to the corresponding region in the precursors of several other structurally unrelated myeloid antimicrobial peptides. Furthermore, a 148 nt non-coding region at the 3' end is 75% homologous to a corresponding region of the cDNA of the precursor of PR-39, a porcine antibacterial peptide which is also proline- and arginine-rich.

引用

页码：197 / 200

页数：4