EXPRESSION OF THE AMINO-TERMINAL HALF OF THE NS1 REGION OF THE HEPATITIS-C VIRUS GENOME AND DETECTION OF AN ANTIBODY TO THE EXPRESSED PROTEIN IN PATIENTS WITH LIVER-DISEASES

被引：29

作者：

INOUE, Y

SUZUKI, R

MATSUURA, Y

HARADA, S

CHIBA, J

WATANABE, Y

SAITO, I

MIYAMURA, T

机构：

[1] NATL INST HLTH,HEPATITIS VIRUSES LAB 2,DEPT ENTEROVIRUSES,2-10-35 KAMIOSAKI,TOKYO 141,JAPAN

[2] NATL INST HLTH,DEPT VET MED,SHINAGAWA KU,TOKYO 141,JAPAN

[3] NATL INST HLTH,DEPT MED ENTOMOL,SHINAGAWA KU,TOKYO 141,JAPAN

[4] ST MARIANNA MED UNIV,SCH MED,KAWASAKI 216,JAPAN

[5] SCI UNIV TOKYO,DEPT BIOL SCI & TECHNOL,NODA,CHIBA 278,JAPAN

[6] UNIV TOKYO,INST MED SCI,MOLEC GENET LAB,MINATO KU,TOKYO 108,JAPAN

来源：

JOURNAL OF GENERAL VIROLOGY | 1992年 / 73卷

关键词：

D O I：

10.1099/0022-1317-73-8-2151

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA fragment encompassing the 5'-terminal half of the NS1 region of the hepatitis C virus (HCV) genome was cloned. The cDNA was expressed in insect cells using a recombinant baculovirus, and a protein band of approximately 21 K was identified by immunoblotting with a serum sample from a patient with chronic hepatitis C. Antibody to the protein was detected in sera from 13.4% of patients with chronic non-A, non-B hepatitis (NANBH), 20.8% of patients with liver cirrhosis and 16.8% of patients with hepatocellular carcinoma with no serum markers for hepatitis B virus infection. However, the antibody was not detected in sera from patients with actute NANBH. The prevalence of antibody to the protein encoded by the NS1 region was lower than that of antibody to the HCV core protein, but much higher than that of antibody to the envelope protein. Thus, the NS1 region of the HCV genome is suggested to encode a protein produced during the course of HCV replication.

引用

页码：2151 / 2154

页数：4

共 36 条

[11] EXAMINATION OF THE IMMUNOLOGICAL RELATIONSHIPS BETWEEN FLAVIVIRUSES USING YELLOW-FEVER VIRUS MONOCLONAL-ANTIBODIES [J].