DETERMINATION OF 3-HYDROXYBUTYRATE IN SERUM BY FLOW-INJECTION ANALYSIS USING A CO-IMMOBILIZED 3-HYDROXYBUTYRATE DEHYDROGENASE NADH OXIDASE REACTOR AND A CHEMILUMINOMETER

被引：6

作者：

KIBA, N

KOEMADO, H

INAGAKI, J

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 298卷 / 01期

关键词：

CHEMILUMINESCENCE; FLOW INJECTION; CO-IMMOBILIZED ENZYMES REACTOR; 3-HYDROXYBUTYRATE;

D O I：

10.1016/0003-2670(94)90051-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system with a co-immobilized enzyme reactor is described for the determination of 3-hydroxybutyrate. 3Hydroxybutyrate dehydrogenase and NADH oxidase were immobilized on aminated poly(vinyl alcohol) beads and packed into a stainless-steel column (5 cm X 4 mm i.d.). Serum was diluted 10-fold with berate buffer (pH 9.4) and ultrafiltered. Filtrate (20 mu l) was injected into the carrier stream. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(III) reaction. The calibration graph was linear for 2x10(-7) M-5 X 10(-4) M; the detection limit was 8 X 10(-8) M and the sample throughput was 30 h(-1) without carryover.

引用

页码：129 / 133

页数：5

共 15 条

[1] A SPECTROPHOTOMETRIC, ENZYMATIC ASSAY FOR D-3-HYDROXYBUTYRATE THAT IS NOT DEPENDENT ON HYDRAZINE [J].