ENZYMATIC SPECTROPHOTOMETRIC DETERMINATION OF PHYTIC ACID WITH PHYTASE FROM ASPERGILLUS-FICUUM

被引：32

作者：

MARCH, JG ^{[1
]}

VILLACAMPA, AI ^{[1
]}

GRASES, F ^{[1
]}

机构：

[1] UNIV BALEARIC ISL,DEPT CHEM,E-07071 PALMA DE MALLORCA,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 300卷 / 1-3期

关键词：

ENZYMATIC METHODS; SPECTROPHOTOMETRY; PHYTIC ACID; ASPERGILLUS FICUUM;

D O I：

10.1016/0003-2670(94)00367-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method to determine phytic acid in the range 3-60 mu M based on the spectrophotometric determination of inorganic phosphate with vanadate and molybdate, after Liberation by enzymatic hydrolysis of phytic acid with phytase from Aspergillus ficuum at pH 2.5 and 37 degrees C is reported. The method has been applied successfully to determine phytic acid in wheat flour and in a pharmaceutical product.

引用

页码：269 / 272

页数：4

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