MATRIX SOLID-PHASE DISPERSION AS A MULTIRESIDUE EXTRACTION TECHNIQUE FOR BETA-AGONISTS IN BOVINE LIVER-TISSUE

A procedure for the extraction of salbutamol including conjugated forms of the drug, from liver samples, is described. It combines matrix solid-phase dispersion with radioimmunoassay for the measurement of salbutamol residues at the sub-ppb level. Inter- and intra-assay validation, carried out on fortified liver samples, show good recoveries over the range 1-5 ppb of salbutamol. An enzyme hydrolysis procedure was optimized for the deconjugation of incurred residue. The developed procedure is shown to be suitable for the extraction and determination of other P-agonists such as clenbuterol, mabuterol, terbutaline, and cimaterol at residue levels of less than 1 ng g(-1).