INSTABILITY OF IMMUNOGLOBULIN GENES IN S107 CELL-LINE

被引：7

作者：

SHIN, SU ^{[1
]}

DEPINHO, R ^{[1
]}

ZACK, DJ ^{[1
]}

RUDIKOFF, S ^{[1
]}

SCHARFF, MD ^{[1
]}

机构：

[1] NCI,BETHESDA,MD 20892

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1991年 / 17卷 / 03期

关键词：

D O I：

10.1007/BF01232821

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Somatic mutation occurs frequently in rearranged and expressed immunoglobulin variable region genes in vivo. In contrast, V region hypermutation seldom occurs in antibody-forming cells in culture. The S107 mouse myeloma cell line is one of the few cell lines that has been observed to generate V region mutations frequently and spontaneously in vitro. Detailed examination reveals that both the S107 tumor and the cell line derived from it contain and express a duplicated heavy-chain gene. In culture, only one of the two heavy-chain genes undergoes both V and C region mutation, and variants with complex phenotypes and genotypes arise as a result of mutation and segregation of these duplicated genes.

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页码：259 / 276

页数：18

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