INVESTIGATION OF THE CONJUGASE TREATMENT PROCEDURE IN THE MICROBIOLOGICAL ASSAY OF FOLATE

被引：21

作者：

GOLI, DM ^{[1
]}

VANDERSLICE, JT ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,BELTSVILLE HUMAN NUTR RES CTR,NUTRIENT COMPOSIT LAB,BELTSVILLE,MD 20705

来源：

FOOD CHEMISTRY | 1992年 / 43卷 / 01期

关键词：

D O I：

10.1016/0308-8146(92)90242-T

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Microbiological assays have been conducted to determine the growth responses of Lactobacillus casei to pteroyl di-, tri-, tetra-, hexa-, and hepta- glutamates relative to the monoglutamate (folic acid). Deconjugation using human blood plasma conjugate at acidic pH (4.5) results in a substantial degradation of folates while between pH 6.0 and 7.0, optimum results could be obtained using a slight excess of plasma. The end products of conjugase treatment with either plasma or chicken pancreas extract as the source of conjugase depend on, besides the conjugase source, the incubation pH and the inhibitory components present in the incubation mixture.

引用

页码：57 / 64

页数：8

共 25 条

[11]

LAKSHMAIAH N, 1975, INT J VITAM NUTR RES, V45, P183

[12]

LAKSHMAIAH N, 1975, INT J VITAM NUTR RES, V45, P262

[13]

Lakshmaiah N, 1980, Methods Enzymol, V66, P670

[14]

LEICHTER J, 1977, P SOC EXP BIOL MED, V154, P98, DOI 10.3181/00379727-154-39612