COMPLETE LARGE-MOLECULE HIGH-RESOLUTION MASS-SPECTRA FROM 50-FEMTOMOLE MICROVOLUME INJECTION

被引：18

作者：

KELLEHER, NL ^{[1
]}

SENKO, MW ^{[1
]}

LITTLE, DP ^{[1
]}

OCONNOR, PB ^{[1
]}

MCLAFFERTY, FW ^{[1
]}

机构：

[1] CORNELL UNIV,BAKER LAB CHEM,ITHACA,NY 14853

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1995年 / 6卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/1044-0305(95)00088-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 x 10(-14) mol (0.5 mu L of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 mu s from a 0.15-mu L/min injection of 20-mu M ubiquitin consumes 5 x 10(-18) mol, which produces a spectrum with 23:1 signal-to-noise ratio and > 3 X 10(5) resolving power.

引用

收藏

页码：220 / 221

页数：2

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