TOTAL SYNTHESIS OF CYCLOISODITYROSINE, RA-VII, DEOXYBOUVARDIN, AND N29-DESMETHYL-RA-VII - IDENTIFICATION OF THE PHARMACOPHORE AND REVERSAL OF THE SUBUNIT FUNCTIONAL ROLES

被引:88
作者
BOGER, DL
YOHANNES, D
ZHOU, JC
PATANE, MA
机构
[1] PURDUE UNIV, DEPT CHEM, W LAFAYETTE, IN 47907 USA
[2] PURDUE UNIV, DEPT MED CHEM, W LAFAYETTE, IN 47907 USA
关键词
D O I
10.1021/ja00062a004
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Full details of a concise total synthesis of RA-VII (1) and deoxybouvardin (2) are described based on the implementation of an effective intramolecular Ullmann reaction as the key macrocyclization reaction in the preparation of the elusive 14-membered cycloisodityrosine subunit (33) of the bicyclic hexapeptides. Subsequent coupling of 34 to tetrapeptide 17 and macrocyclization with C2-N3 amide bond formation provided 1 and 2. In efforts that address the key structural and conformational features of the agents that contribute to their antitumor activity, N29-desmethyl-RA-VII was prepared and its chemical, conformational, and preliminary biological properties are detailed. The comparable conformational features of N29-desmethyl-RA-VII and RA-VII including a characteristic cis C30-N29 amide bond suggest that the tetrapeptide housed within the 18-membered ring induces the 14-membered cycloisodityrosine to adopt a conformation possessing an inherently disfavored cis secondary or tertiary amide. Moreover, in contrast to prior suppositions in which the rigid 14-membered ring of N-methylcycloisodityrosine has been suggested to serve the functional role of inducing a rigid, normally inaccessible conformation within the biologically relevant D-Ala-Ala-N-Me-Tyr-(OMe)-Ala tetrapeptide, experimental studies demonstrating that the intrinsic activity of the agents resides within the cycloisodityrosine subunit are presented. Thus, the results of the experimental studies require a reversal of the functional roles of the subunits of the agents in which it is the tetrapeptide housed within the 18-membered ring that potentiates the inherent biological properties and alters the conformation of cycloisodityrosine.
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页码:3420 / 3430
页数:11
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