利用基质辅助激光解吸电离飞行时间质谱快速鉴定产气荚膜梭菌

被引：6

作者：

汪琦

王紫薇

赵晓娟

李丹

曹嘉悦

马丹

曾静

机构：

[1] 北京检验检疫技术中心

来源：

食品与发酵工业 | 2018年 / 44卷 / 10期

基金：

国家重点研发计划;

关键词：

产气荚膜梭菌; 基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF MS); 16S rRNA; VITEK; 鉴定;

D O I：

10.13995/j.cnki.11-1802/ts.017269

中图分类号：

O657.63 [质谱分析]; TS252.7 [产品标准与检验];

学科分类号：

摘要：

分别采用基质辅助激光解吸电离飞行时间质谱方法(matrix-assisted laser desorption/ionization time-offlight mass spectrometry,MALDI-TOF MS)、16S rRNA基因测序、VITEK 2 COMPACT和传统生化方法(牛乳汹涌发酵实验)鉴定实验室保存的51株产气荚膜梭菌,以评估MALDI-TOF MS方法是否适用于产气荚膜梭菌的快速鉴定。MALDI-TOF MS方法将51株菌均鉴定为产气荚膜梭菌且鉴定分值在2. 300以上,达到鉴定到种的要求。16S rRNA基因序列比对结果与MALDI-TOF MS方法结果一致。VITEK 2 COMPACT将49株菌鉴定为产气荚膜梭菌,2株菌未能成功鉴定。47株菌的牛乳汹涌发酵实验呈阳性,4株菌为牛乳汹涌发酵实验阴性,且这4株菌经其他3种方法均鉴定为产气荚膜梭菌。结果表明,无论是牛乳汹涌发酵实验还是VITEK 2 COMPACT,在检测产气荚膜梭菌时都有可能发生漏检。相比牛乳汹涌发酵实验、VITEK 2 COMPACT和16S rRNA测序方法,MALDI-TOF MS方法更加快速、准确和低成本,适用于产气荚膜梭菌的快速鉴定。

引用

页码：219 / 224

页数：6

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