蛋白质突变体基因库构建方法的研究进展

被引：5

作者：

张秀艳 ^{[1
]}

何国庆 ^{[2
]}

机构：

[1] 华中农业大学食品化学与分子生物学系

[2] 浙江大学食品科学与营养系

来源：

中国生物工程杂志 | 2006年 / 10期

关键词：

定向进化; 基因文库; 蛋白质工程; 计算设计方法; 理性设计;

D O I：

10.13523/j.cb.20061011

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

体外定向进化是蛋白质工程中一个非常有效的设计策略。最近几年,在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。对这些方法及其特点加以总结,可为解决特定问题选取相应方法提供一定依据。最近研究表明:定向进化和理性设计相结合、定向进化和以结构为基础的计算设计方法相结合正成为蛋白质工程中两个新的发展方向。

引用

页码：52 / 58

页数：7

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