纳米羟基磷灰石/壳聚糖酶促物理交联水凝胶的制备及性能评价

被引：7

作者：

闫香珍

夏思颖

罗礼君

机构：

[1] 同济大学口腔医学院和附属口腔医院牙周病科(上海牙组织修复与再生工程技术研究中心)

来源：

江苏医药 | 2019年 / 45卷 / 11期

关键词：

壳聚糖; 水凝胶; 纳米羟基磷灰石; 牙周膜细胞;

D O I：

10.19460/j.cnki.0253-3685.2019.11.002

中图分类号：

R318.08 [生物材料学];

学科分类号：

100103 [病原生物学];

摘要：

目的制备纳米羟基磷灰石(nHA)/壳聚糖(CS)酶促物理交联水凝胶,并对其性能进行体外评价。方法在CS溶液中加入0.75%、1.5%和3%(W/V)nHA(A1、A2和A3组),制备不同浓度的nHA/CS酶促物理交联水凝胶,另设不含nHA的CS溶液为对照(C组),并对其凝胶时间、形貌结构及体外成骨诱导能力进行评价。结果 A2和A3组比C组的凝胶时间更短[(158±8) s和(152±9) s vs.(204±13)s](P<0.05)。nHA/CS酶促物理交联水凝胶粗糙表面可观察到nHA微粒。与C组比较,A1、A2和A3组牙周膜细胞钙盐沉积量均增加(P<0.05),且A3组高于A1、A2组(P<0.05)。1周后,与C组比较,A1、A2和A3组在模拟体液中的钙吸收量均增加(P<0.05),且A2、A3组高于A1组(P<0.05)。结论 nHA/CS酶促物理交联水凝胶可原位成胶,具有良好的生物相容性及生物活性。

引用

页码：1085 / 1088+1076 +1076

页数：5

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