The gross structure of the respiratory complex I:: a Lego System

被引:107
作者
Friedrich, T
Böttcher, B
机构
[1] Univ Freiburg, Inst Organ Chem & Biochem, D-79104 Freiburg, Germany
[2] European Mol Biol Lab, Struct & Computat Biol Programme, D-69117 Heidelberg, Germany
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2004年 / 1608卷 / 01期
关键词
NADH : ubiquinone oxidoreductase; complex I; electron microscopy; single particle analysis; image processing; protein structure; modular evolution;
D O I
10.1016/j.bbabio.2003.10.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The proton-pumping NADH:ubiquinone oxidoreductase, also called complex 1, is the entry point for electrons into the respiratory chains of many bacteria and mitochondria of most eucaryotes. It couples electron transfer with the translocation of protons across the membrane, thus providing the proton motive force essential for energy-consuming processes. Electron microscopy revealed the 'L'-shaped structure of the bacterial and mitochondrial complex with two arms arranged perpendicular to each other. Recently, we showed that the Escherichia coli complex I takes on another stable conformation with the two arms arranged side by side resulting in a horseshoe-shaped structure. This model reflects the evolution of complex I from pre-existing modules for electron transfer and proton translocation. (C) 2003 Elsevier B.V. All rights reserved.
引用
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页码:1 / 9
页数:9
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