Virus-Mimetic Fusogenic Exosomes for Direct Delivery of Integral Membrane Proteins to Target Cell Membranes

被引：138

作者：

Yang, Yoosoo ^{[1
]}

Hong, Yeonsun ^{[1
,2
]}

Nam, Gi-Hoon ^{[1
,2
]}

Chung, Jin Hwa ^{[3
]}

Koh, Eunee ^{[1
,2
]}

Kim, In-San ^{[1
,2
]}

机构：

[1] Korea Inst Sci & Technol, Biomed Res Inst, Seoul 02792, South Korea

[2] Korea Univ, KU KIST, Grad Sch Converging Sci & Technol, Seoul 02841, South Korea

[3] Asan Inst Life Sci, Asan Med Ctr, Bio Imaging Ctr, Seoul 05505, South Korea

来源：

ADVANCED MATERIALS | 2017年 / 29卷 / 13期

关键词：

EXTRACELLULAR VESICLES; FUSION ACTIVITY; CANCER-CELLS; MUSCLE PH; EXERCISE; PEPTIDE; DISEASE;

D O I：

10.1002/adma.201605604

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

An efficient system for direct delivery of integral membrane proteins is successfully developed using a new biocompatible exosome-based platform. Fusogenic exosomes harboring viral fusogen, vascular stomatitis virus (VSV)-G protein, can fuse with and modify plasma membranes in a process called "membrane editing." This can facilitate the transfer of biologically active membrane proteins into the target cell membranes both in vitro and in vivo.

引用

页数：7

共 44 条

[1]

Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes [J].

Alvarez-Erviti, Lydia ;

Seow, Yiqi ;

Yin, HaiFang ;

Betts, Corinne ;

Lakhal, Samira ;

Wood, Matthew J. A. .

NATURE BIOTECHNOLOGY, 2011, 29 (04) :341-U179

[2]

Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor [J].

Amirache, Fouzia ;

Levy, Camille ;

Costa, Caroline ;

Mangeot, Philippe-Emmanuel ;

Torbett, Bruce E. ;

Wang, Cathy X. ;

Negre, Didier ;

Cosset, Francois-Loic ;

Verhoeyen, Els .

BLOOD, 2014, 123 (09) :1422-1424

[3]

Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion [J].

Biner, Olivier ;

Schick, Thomas ;

Muller, Yannic ;

von Ballmoos, Christoph .

FEBS LETTERS, 2016, 590 (14) :2051-2062

[4]

Bruno BJ, 2013, THER DELIV, V4, P1443, DOI [10.4155/TDE.13.104, 10.4155/tde.13.104]

[5]

Complex adaptive therapeutic strategy (CATS) for cancer [J].

Cho, Yong Woo ;

Kim, Sang Yoon ;

Kwon, Ick Chan ;

Kim, In-San .

JOURNAL OF CONTROLLED RELEASE, 2014, 175 :43-47

[6]

Aberrant trafficking of transmembrane proteins in human disease [J].

Cobbold, C ;

Monaco, AP ;

Sivaprasadarao, A ;

Ponnambalam, S .

TRENDS IN CELL BIOLOGY, 2003, 13 (12) :639-647

[7]

The Future of Peptide-based Drugs [J].

Craik, David J. ;

Fairlie, David P. ;

Liras, Spiros ;

Price, David .

CHEMICAL BIOLOGY & DRUG DESIGN, 2013, 81 (01) :136-147

[8]

Altering the tropism of lentiviral vectors through pseudotyping [J].

Cronin, J ;

Zhang, XY ;

Reiser, J .

CURRENT GENE THERAPY, 2005, 5 (04) :387-398

[9]

Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion [J].

de Planque, M. R. R. ;

Mendes, G. P. ;

Zagnoni, M. ;

Sandison, M. E. ;

Fisher, K. H. ;

Berry, R. M. ;

Watts, A. ;

Morgan, H. .

IEE PROCEEDINGS-NANOBIOTECHNOLOGY, 2006, 153 (02) :21-30

[10]

A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins [J].

Diao, Jiajie ;

Ishitsuka, Yuji ;

Lee, Hanki ;

Joo, Chirlmin ;

Su, Zengliu ;

Syed, Salman ;

Shin, Yeon-Kyun ;

Yoon, Tae-Young ;

Ha, Taekjip .

NATURE PROTOCOLS, 2012, 7 (05) :921-934

← 1 2 3 4 5 →