Simultaneous Imaging of Mitochondria and Lysosomes by Using Two-Photon Fluorescent Probes

被引：44

作者：

Han, Ji Hee ^{[2
]}

Park, Sun Keun ^{[1
]}

Lim, Chang Su ^{[2
]}

Park, Min Kyu ^{[2
]}

Kim, Hyung Joong ^{[1
]}

Kim, Hwan Myung ^{[1
]}

Cho, Bong Rae ^{[2
]}

机构：

[1] Ajou Univ, Div Energy Syst Res, Suwon 443749, South Korea

[2] Korea Univ, Dept Chem, Seoul 136701, South Korea

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2012年 / 18卷 / 48期

基金：

新加坡国家研究基金会;

关键词：

fluorescence; fluorescent probes; imaging agents; lysosomes; mitochondria; HYDROGEN-PEROXIDE; AUTOPHAGY; SUPERIOR;

D O I：

10.1002/chem.201203452

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：15246 / 15249

页数：4

共 31 条

[1] Quantifying Colocalization by Correlation: The Pearson Correlation Coefficient is Superior to the Mander's Overlap Coefficient [J].