Production of octadecylphospho-L-serine by phospholipase D

被引：5

作者：

Aurich, I ^{[1
]}

Dürrschmidt, P ^{[1
]}

Schierhorn, A ^{[1
]}

Ulbrich-Hofmann, R ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Dept Biochem Biotechnol, D-06099 Halle Saale, Germany

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 07期

关键词：

alkylphosphate esters; alkylphospholipids; enzymatic transesterification; octadecylphospho-L-serine; phospholipase D;

D O I：

10.1023/A:1014876914801

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Octadecylphospho-L-serine (OPS), belonging to the family of alkylphosphate esters with anticancer activity, was synthesized by transesterification of octadecylphosphocholine using phospholipase D (PLD). With respect to the yield of product, PLD from cabbage proved to be superior to PLD from Streptomyces spp. Although the addition of n-hexane/2-octanol is advantageous to suppress the hydrolytic byproduct, a purely aqueous reaction medium was preferred because of better recovery of the product.

引用

页码：585 / 590

页数：6

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