Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection

被引：17

作者：

Angeles Orellana-Miguel, Ma ^{[1
]}

Alcolea-Medina, Adela ^{[1
]}

Barrado-Blanco, Laura ^{[1
]}

Rodriguez-Otero, Joaquin ^{[1
]}

Chaves-Sanchez, Fernando ^{[1
]}

机构：

[1] Hosp Univ 12 Octubre, Microbiol Serv, Madrid, Spain

来源：

ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2013年 / 31卷 / 02期

关键词：

Clostridium difficile infection; Nosocomial diarrhoea; Toxigenic Clostridium difficile; GLUTAMATE-DEHYDROGENASE; TOXIN; DIAGNOSIS; ASSAY; CULTURE; SOCIETY; TESTS;

D O I：

10.1016/j.eimc.2012.01.003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objective: To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C difficile infection (CDI) based on this test. Methods: We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference. Results: This test enabled us to obtain a conclusive result in <30 min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C dfficile by toxigenic culture. Conclusion: This test has a high sensitivity and specificity for the diagnosis of CDI. (C) 2011 Elsevier Espana, S.L. All rights reserved.

引用

页码：97 / 99

页数：3

共 13 条

[1] A thermally sensitive loop in clostridial glutamate dehydrogenase detected by limited proteolysis [J].

Aghajanian, S ;

Hovsepyan, M ;

Geoghegan, KF ;

Chirunyk, BA ;

Engel, PC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (02) :1067-1074

[2] Clinical Practice Guidelines for Clostridium difficile Infection in Adults: 2010 Update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA) [J].

Cohen, Stuart H. ;

Gerding, Dale N. ;

Johnson, Stuart ;

Kelly, Ciaran P. ;

Loo, Vivian G. ;

McDonald, L. Clifford ;

Pepin, Jacques ;

Wilcox, Mark H. .

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2010, 31 (05) :431-455

[3] European Society of Clinical Microbiology and Infectious Diseases (ESCMID): Data review and recommendations for diagnosing Clostridium difficile-infection (CDI) [J].

Crobach, M. J. T. ;

Dekkers, O. M. ;

Wilcox, M. H. ;

Kuijper, E. J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2009, 15 (12) :1053-1066

[4] Rapid and reliable diagnostic algorithm for detection of Clostridium difficile [J].

Fenner, Lukas ;

Widmer, Andreas F. ;

Goy, Gisela ;

Rudin, Sonja ;

Frei, Reno .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :328-330

[5] Two-step glutamate dehydrogenase antigen real-time polymerase chain reaction assay for detection of toxigenic Clostridium difficile [J].

Goldenberg, S. D. ;

Cliff, P. R. ;

Smith, S. ;

Milner, M. ;

French, G. L. .

JOURNAL OF HOSPITAL INFECTION, 2010, 74 (01) :48-54

[6] Comparison of BD GeneOhm Cdiff Real-Time PCR Assay with a Two-Step Algorithm and a Toxin A/B Enzyme-Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection [J].

Kvach, Elizabeth J. ;

Ferguson, David ;

Riska, Paul F. ;

Landry, Marie L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :109-114

[7] C. Diff Quik Chek Complete Enzyme Immunoassay Provides a Reliable First-Line Method for Detection of Clostridium difficile in Stool Specimens [J].

Quinn, Criziel D. ;

Sefers, Susan E. ;

Babiker, Wisal ;

He, Ying ;

Alcabasa, Romina ;

Stratton, Charles W. ;

Carroll, Karen C. ;

Tang, Yi-Wei .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (02) :603-605

[8] Comparison of Two Rapid Assays for Clostridium difficile Common Antigen and a C difficile Toxin A/B Assay With the Cell Culture Neutralization Assay [J].

Reller, Megan E. ;

Alcabasa, Romina C. ;

Lema, Clara A. ;

Carroll, Karen C. .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2010, 133 (01) :107-109

[9] Clostridium difficile testing algorithms: What is practical and feasible? [J].

Schmidt, Monica L. ;

Gilligan, Peter H. .

ANAEROBE, 2009, 15 (06) :270-273

[10] Evaluation of the C. Diff Quik Chek Complete Assay, a New Glutamate Dehydrogenase and A/B Toxin Combination Lateral Flow Assay for Use in Rapid, Simple Diagnosis of Clostridium difficile Disease [J].

Sharp, Susan E. ;

Ruden, Lila O. ;

Pohl, Julie C. ;

Hatcher, Patricia A. ;

Jayne, Linda M. ;

Ivie, W. Michael .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (06) :2082-2086

← 1 2 →