Impedance Biosensor for Peanut Protein Ara h 1

被引:93
作者
Huang, Yin
Bell, Melissa C.
Suni, Ian I. [1 ]
机构
[1] Clarkson Univ, Dept Chem & Biomol Engn, Potsdam, NY 13699 USA
关键词
D O I
10.1021/ac801048g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A reagentless electrochemical impedance biosensor for detection of peanut protein Ara h 1, one of the allergenic proteins found in peanuts, has been demonstrated using an Au substrate onto which an antibody film has been immobilized. Following initial stabilization of the self-assembled monolayer (SAM) through which the antibody is immobilized, the biosensor substrate exhibits stable impedance spectra at different stages of substrate preparation. By fitting the impedance spectra to a Randles equivalent circuit, one can demonstrate that the charge-transfer resistance (R-ct) increases and the differential capacitance (C-d) decreases with increasing concentration of Ara h 1, although R-ct exhibits greater sensitivity. The detection limit of this reagentless biosensor is estimated to be less than 0.3 nM. Assuming a Langmuir adsorption isotherm, the dissociation constant of the peanut protein Ara h 1 and its antibody can be calculated as 0.52 nM from the variation in differential capacitance with Ara h 1 concentration.
引用
收藏
页码:9157 / 9161
页数:5
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