Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

被引：33

作者：

De Medici, D ^{[1
]}

Croci, L ^{[1
]}

Di Pasquale, S ^{[1
]}

Fiore, A ^{[1
]}

Toti, L ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Alimenti, I-00161 Rome, Italy

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 33卷 / 05期

关键词：

D O I：

10.1046/j.1472-765X.2001.01018.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: The objective of this study was to determine the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analysed for the presence of viral HAV-RNA using RT-nested-PCR; positive samples were then analysed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were positive for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to determine the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.

引用

页码：362 / 366

页数：5

共 38 条

[1] Arnal C, 1998, ZBL HYG UMWELTMED, V201, P279
[2] DETECTION OF ENTERIC VIRUSES IN OYSTERS BY USING THE POLYMERASE CHAIN-REACTION
ATMAR, RL
METCALF, TG
NEILL, FH
ESTES, MK
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (02) : 631 - 635
[3] COMPLETE NUCLEOTIDE-SEQUENCE OF A CYTOPATHIC HEPATITIS-A VIRUS-STRAIN ISOLATED IN ITALY
BENEDUCE, F
PISANI, G
DIVIZIA, M
PANA, A
MORACE, G
[J]. VIRUS RESEARCH, 1995, 36 (2-3) : 299 - 309
[4] CICCOZZI M, 1999, 1 C SIMVIM SOC IT ME, P44
[5] METHODS TO DETECT VIRUSES IN FOODS - TESTING AND INTERPRETATION OF RESULTS
CLIVER, DO
ELLENDER, RD
SOBSEY, MD
[J]. JOURNAL OF FOOD PROTECTION, 1983, 46 (04) : 345 - 357
[6] COMPLETE NUCLEOTIDE-SEQUENCE OF WILD-TYPE HEPATITIS-A VIRUS - COMPARISON WITH DIFFERENT STRAINS OF HEPATITIS-A VIRUS AND OTHER PICORNAVIRUSES
COHEN, JI
TICEHURST, JR
PURCELL, RH
BUCKLERWHITE, A
BAROUDY, BM
[J]. JOURNAL OF VIROLOGY, 1987, 61 (01) : 50 - 59
[7] Detection of hepatitis A virus in shellfish by nested reverse transcription-PCR
Croci, L
De Medici, D
Morace, G
Fiore, A
Scalfaro, C
Beneduce, F
Toti, L
[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 48 (01) : 67 - 71
[8] Determination of enteroviruses, hepatitis A virus, bacteriophages and Escherichia coli in Adriatic Sea mussels
Croci, L
De Medici, D
Scalfaro, C
Fiore, A
Divizia, M
Donia, D
Cosentino, AM
Moretti, P
Costantini, G
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (02) : 293 - 298
[9] CROCI L, 1998, P 4 WORLD C FOODB IN, P797
[10] Detection of hepatitis A virus RNA in oyster meat
Cromeans, TL
Nainan, OV
Margolis, HS
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (06) : 2460 - 2463

← 1 2 3 4 →