Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction

被引：57

作者：

Suprun, Elena ^{[1
]}

Shurnyantseva, Victoria ^{[1
]}

Bulko, Tatiana ^{[1
]}

Rachmetova, Svetlana ^{[1
]}

Rad'ko, Sergei ^{[1
]}

Bodoev, Nikolay ^{[1
]}

Archakov, Alexander ^{[1
]}

机构：

[1] Russian Acad Med Sci, Inst Biomed Chem, Moscow 119121, Russia

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

关键词：

Gold-nanoparticles; Aptamers; Thrombin; Stripping voltammetry; Electrochemical aptasensor;

D O I：

10.1016/j.bios.2008.07.008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nano particles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9) M. Linear range corresponds from 10(-8) to 10(-5) M thrombin concentration or 2 x 10(-14) to 2 x 10(-11) mol/electrode (R = 0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator. (C) 2008 Published by Elsevier B.V.

引用

页码：825 / 830

页数：6

共 44 条

[21] Electrochemical DNA sensor for detection of single nucleotide polymorphisms [J].

Marques, LPJ ;

Cavaco, I ;

Pinheiro, JP ;

Ribeiro, V ;

Ferreira, GNM .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2003, 41 (04) :475-481

[22] Development of biosensors with aptamers as bio-recognition element: the case of HIV-1 Tat protein [J].

Minunni, M ;

Tombelli, S ;

Gullotto, A ;

Luzi, E ;

Mascini, M .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (06) :1149-1156

[23] Different strategies to develop an electrochemical thrombin aptasensor [J].

Mir, M ;

Vreeke, M ;

Katakis, L .

ELECTROCHEMISTRY COMMUNICATIONS, 2006, 8 (03) :505-511

[24]

MURRAY RW, 1984, ELECTROANAL CHEM, V13, P191

[25] Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions [J].

Nagatoishi, Satoru ;

Tanaka, Yoshikazu ;

Tsumoto, Kouhei .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 352 (03) :812-817

[26] Nucleic acid aptamers and enzymes as sensors [J].

Navani, Naveen K. ;

Li, Yingfu .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (03) :272-281

[27] Electrochemical genosensor based on colloidal gold nanoparticles for the detection of Factor V Leiden mutation using disposable pencil graphite electrodes [J].

Ozsoz, M ;

Erdem, A ;

Kerman, K ;

Ozkan, D ;

Tugrul, B ;

Topcuoglu, N ;

Ekren, H ;

Taylan, M .

ANALYTICAL CHEMISTRY, 2003, 75 (09) :2181-2187

[28] Direct voltammetric determination of gold nanoparticles using graphite-epoxy composite electrode [J].

Pumera, M ;

Aldavert, M ;

Mills, C ;

Merkoçi, A ;

Alegret, S .

ELECTROCHIMICA ACTA, 2005, 50 (18) :3702-3707

[29] Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor [J].

Radi, AE ;

Sánchez, JLA ;

Baldrich, E ;

O'Sullivan, CK .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (01) :117-124

[30] ENHANCED ELECTRON-TRANSFER FOR MYOGLOBIN IN SURFACTANT FILMS ON ELECTRODES [J].

RUSLING, JF ;

NASSAR, AEF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (25) :11891-11897

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