Preparation of Cationic Nanogels for Nucleic Acid Delivery

被引：66

作者：

Averick, Saadyah E. ^{[1
]}

Paredes, Eduardo ^{[2
]}

Irastorza, Ainara ^{[3
]}

Shrivats, Arun R. ^{[3
]}

Srinivasan, Abiraman ^{[1
]}

Siegwart, Daniel J. ^{[1
]}

Magenau, Andrew J. ^{[1
]}

Cho, Hong Y. ^{[1
]}

Hsu, Eric ^{[3
]}

Averick, Amram A. ^{[1
]}

Kim, Jinku ^{[3
]}

Liu, Shiguang ^{[3
]}

Hollinger, Jeffrey O. ^{[3
]}

Das, Subha R. ^{[2
]}

Matyjaszewski, Krzysztof ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[2] Carnegie Mellon Univ, Ctr Nucle Acids Sci & Technol, Pittsburgh, PA 15213 USA

[3] Carnegie Mellon Univ, Dept Biomed Engn & Biol Sci, Bone Tissue Engn Ctr, Pittsburgh, PA 15219 USA

来源：

BIOMACROMOLECULES | 2012年 / 13卷 / 11期

基金：

美国国家科学基金会;

关键词：

TRANSFER RADICAL POLYMERIZATION; GENE DELIVERY; SIRNA DELIVERY; STAR POLYMERS; PROTEIN; DRUG; ATRP; RNA; DNA; NANOPARTICLES;

D O I：

10.1021/bm301166s

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cationic nanogels with, site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2,(dimethylamino)-ethyl methacrylate and a cross linker with reducible disulfide moieties.(qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid. DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated The,delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.

引用

页码：3445 / 3449

页数：5

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