Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation

被引：24

作者：

Betzenhauser, Matthew J. ^{[1
]}

Wagner, Larry E., II ^{[1
]}

Won, Jong Hak ^{[1
]}

Yule, David I. ^{[1
]}

机构：

[1] Univ Rochester, Dept Physiol & Pharmacol, Rochester, NY 14642 USA

来源：

METHODS | 2008年 / 46卷 / 03期

关键词：

Ca2+ release; Inositol 1,4,5-trisphosphate receptors; DT40-3KO cells; Permeabilized cells; Flash photolysis;

D O I：

10.1016/j.ymeth.2008.09.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inositol 1,4,5-trisphosphate receptors (InsP(3)R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP(3)R family members with particular reference to methods applicable following expression of recombinant InsP(3)R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP(3)R expression system. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：177 / 182

页数：6

共 44 条

[1] Lateral inhibition of inositol 1,4,5-trisphosphate receptors by cytosolic Ca2+ [J].

Adkins, CE ;

Taylor, CW .

CURRENT BIOLOGY, 1999, 9 (19) :1115-1118

[2] Protein kinase C phosphorylates the inositol 1,4,5-trisphosphate receptor type 2 and decreases the mobilization of Ca2+ in pancreatoma AR4-2J cells [J].

Arguin, Guillaume ;

Regimbald-Dumas, Yannik ;

Fregeau, Marc-Olivier ;

Caron, Annabelle Z. ;

Guillemette, Gaetan .

JOURNAL OF ENDOCRINOLOGY, 2007, 192 (03) :659-668

[3] Protein kinase A increases the binding affinity and the Ca2+ release activity of the inositol 1,4,5-trisphosphate receptor type 3 in RINm5F cells [J].

Chaloux, Benoit ;

Caron, Annabelle Z. ;

Guillemette, Gaetan .

BIOLOGY OF THE CELL, 2007, 99 (07) :379-388

[4] Cell membrane permeable esters of D-myo-inositol 1,4,5-trisphosphate [J].

Dakin, Kenneth ;

Li, Wen-Hong .

CELL CALCIUM, 2007, 42 (03) :291-301

[5] INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS - DISTINCT NEURONAL AND NONNEURONAL FORMS DERIVED BY ALTERNATIVE SPLICING DIFFER IN PHOSPHORYLATION [J].

DANOFF, SK ;

FERRIS, CD ;

DONATH, C ;

FISCHER, GA ;

MUNEMITSU, S ;

ULLRICH, A ;

SNYDER, SH ;

ROSS, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (07) :2951-2955

[6]

DESMEDT H, 1994, J BIOL CHEM, V269, P21691

[7]

DeSmedt H, 1997, BIOCHEM J, V322, P575

[8] Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum [J].

Echevarria, W ;

Leite, MF ;

Guerra, MT ;

Zipfel, WR ;

Nathanson, MH .

NATURE CELL BIOLOGY, 2003, 5 (05) :440-446

[9] Inositol trisphosphate receptor Ca2+ release channels [J].

Foskett, J. Kevin ;

White, Carl ;

Cheung, King-Ho ;

Mak, Don-On Daniel .

PHYSIOLOGICAL REVIEWS, 2007, 87 (02) :593-658

[10] Reduced IP3 sensitivity of IP3 receptor in Purkinje neurons [J].

Fujiwara, A ;

Hirose, K ;

Yamazawa, T ;

Lino, M .

NEUROREPORT, 2001, 12 (12) :2647-2651

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