Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation

被引：24

作者：

Betzenhauser, Matthew J. ^{[1
]}

Wagner, Larry E., II ^{[1
]}

Won, Jong Hak ^{[1
]}

Yule, David I. ^{[1
]}

机构：

[1] Univ Rochester, Dept Physiol & Pharmacol, Rochester, NY 14642 USA

来源：

METHODS | 2008年 / 46卷 / 03期

关键词：

Ca2+ release; Inositol 1,4,5-trisphosphate receptors; DT40-3KO cells; Permeabilized cells; Flash photolysis;

D O I：

10.1016/j.ymeth.2008.09.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inositol 1,4,5-trisphosphate receptors (InsP(3)R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP(3)R family members with particular reference to methods applicable following expression of recombinant InsP(3)R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP(3)R expression system. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：177 / 182

页数：6

共 44 条

[41] Phosphorylation of type-1 inositol 1,4,5-trisphosphate receptors by cyclic nucleotide-dependent protein kinases -: A mutational analysis of the functionally important sites in the S2+ and S2- splice variants [J].

Wagner, LE ;

Li, WH ;

Yule, DI .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (46) :45811-45817

[42] TYPE-I, TYPE-II, AND TYPE-III INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS ARE UNEQUALLY SUSCEPTIBLE TO DOWN-REGULATION AND ARE EXPRESSED IN MARKEDLY DIFFERENT PROPORTIONS IN DIFFERENT CELL-TYPES [J].

WOJCIKIEWICZ, RJH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (19) :11678-11683

[43] Phosphorylation of inositol 1,4,5-trisphosphate receptors by cAMP-dependent protein kinase - Type I, II and III receptors are differentially susceptible to phosphorylation and are phosphorylated in intact cells [J].

Wojcikiewicz, RJH ;

Luo, SG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (10) :5670-5677

[44] Ca2+ release dynamics in parotid and pancreatic exocrine acinar cells evoked by spatially limited flash photolysis [J].

Won, Jong Hak ;

Cottrell, William J. ;

Foster, Thomas H. ;

Yule, David I. .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2007, 293 (06) :G1166-G1177

← 1 2 3 4 5 →