Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation

被引：24

作者：

Betzenhauser, Matthew J. ^{[1
]}

Wagner, Larry E., II ^{[1
]}

Won, Jong Hak ^{[1
]}

Yule, David I. ^{[1
]}

机构：

[1] Univ Rochester, Dept Physiol & Pharmacol, Rochester, NY 14642 USA

来源：

METHODS | 2008年 / 46卷 / 03期

关键词：

Ca2+ release; Inositol 1,4,5-trisphosphate receptors; DT40-3KO cells; Permeabilized cells; Flash photolysis;

D O I：

10.1016/j.ymeth.2008.09.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inositol 1,4,5-trisphosphate receptors (InsP(3)R) are a family of ubiquitously expressed intracellular Ca2+ channels. Isoform-specific properties of the three family members may play a prominent role in defining the rich diversity of the spatial and temporal characteristics of intracellular Ca2+ signals. Studying the properties of the particular family members is complicated because individual receptor isoforms are typically never expressed in isolation. In this article, we discuss strategies for studying Ca2+ release through individual InsP(3)R family members with particular reference to methods applicable following expression of recombinant InsP(3)R and mutant constructs in the DT40-3KO cell line, an unambiguously null InsP(3)R expression system. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：177 / 182

页数：6

共 44 条

[11] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE INOSITOL 1,4,5-TRISPHOSPHATE-BINDING PROTEIN-P400 [J].

FURUICHI, T ;

YOSHIKAWA, S ;

MIYAWAKI, A ;

WADA, K ;

MAEDA, N ;

MIKOSHIBA, K .

NATURE, 1989, 342 (6245) :32-38

[12] Type III InsP3 receptor channel stays open in the presence of increased calcium [J].

Hagar, RE ;

Burgstahler, AD ;

Nathanson, MH ;

Ehrlich, BE .

NATURE, 1998, 396 (6706) :81-84

[13] Minimal requirements for calcium oscillations driven by the IP3 receptor [J].

Hajnoczky, G ;

Thomas, AP .

EMBO JOURNAL, 1997, 16 (12) :3533-3543

[14] Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling [J].

Hattori, M ;

Suzuki, AZ ;

Higo, T ;

Miyauchi, H ;

Michikawa, T ;

Nakamura, T ;

Inoue, T ;

Mikoshiba, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (12) :11967-11975

[15] The type II inositol 1,4,5-trisphosphate receptor can trigger Ca2+ waves in rat hepatocytes [J].

Hirata, K ;

Pusl, T ;

O'Neill, AF ;

Dranoff, JA ;

Nathanson, MH .

GASTROENTEROLOGY, 2002, 122 (04) :1088-1100

[16]

HIROSE K, 1994, NATURE, V372, P791

[17]

Hofer Aldebaran M, 2006, Methods Mol Biol, V312, P229, DOI 10.1385/1-59259-949-4:229

[18] Quantification of intraluminal free [Ca] in the agonist-sensitive internal calcium store using compartmentalized fluorescent indicators: Some considerations [J].

Hofer, AM ;

Schulz, I .

CELL CALCIUM, 1996, 20 (03) :235-242

[19] TECHNIQUE FOR INSITU MEASUREMENT OF CALCIUM IN INTRACELLULAR INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE STORES USING THE FLUORESCENT INDICATOR MAG-FURA-2 [J].

HOFER, AM ;

MACHEN, TE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2598-2602

[20] Molecular cloning of mouse type 2 and type 3 inositol 1,4,5-trisphosphate receptors and identification of a novel type 2 receptor splice variant [J].

Iwai, M ;

Tateishi, Y ;

Hattori, M ;

Mizutani, A ;

Nakamura, T ;

Futatsugi, A ;

Inoue, T ;

Furuichi, T ;

Michikawa, T ;

Mikoshiba, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (11) :10305-10317

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