Introns of plant pri-miRNAs enhance miRNA biogenesis

被引：92

作者：

Bielewicz, Dawid ^{[1
]}

Kalak, Malgorzata ^{[1
]}

Kalyna, Maria ^{[2
]}

Windels, David ^{[3
]}

Barta, Andrea ^{[2
]}

Vazquez, Franck ^{[3
]}

Szweykowska-Kulinska, Zofia ^{[1
]}

Jarmolowski, Artur ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Fac Biol, Dept Gene Express, PL-61614 Poznan, Poland

[2] Univ Vienna, Max F Perutz Labs, A-1030 Vienna, Austria

[3] Univ Basel, Zurich Basel Plant Sci Ctr, Inst Bot, Swiss Plant Sci Web, CH-4056 Basel, Switzerland

来源：

EMBO REPORTS | 2013年 / 14卷 / 07期

基金：

瑞士国家科学基金会; 奥地利科学基金会;

关键词：

plant; Arabidopsis; RNA; splicing; miRNA; BINDING-PROTEIN HYL1; ARABIDOPSIS-THALIANA; MESSENGER-RNA; MICRORNAS; GENES; ACCUMULATION; PATHWAYS; CLEAVAGE; TARGETS; ROLES;

D O I：

10.1038/embor.2013.62

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant MIR genes are independent transcription units that encode long primary miRNA precursors, which usually contain introns. For two miRNA genes, MIR163 and MIR161, we show that introns are crucial for the accumulation of proper levels of mature miRNA. Removal of the intron in both cases led to a drop-off in the level of mature miRNAs. We demonstrate that the stimulating effects of the intron mostly reside in the 5'ss rather than on a genuine splicing event. Our findings are biologically significant as the presence of functional splice sites in the MIR163 gene appears mandatory for pathogen-triggered accumulation of miR163 and proper regulation of at least one of its targets.

引用

页码：622 / 628

页数：7

共 29 条

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