Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes

被引:118
作者
Blower, Tim R. [1 ]
Short, Francesca L. [1 ]
Rao, Feng [1 ]
Mizuguchi, Kenji [2 ]
Pei, Xue Y. [1 ]
Fineran, Peter C. [3 ]
Luisi, Ben F. [1 ]
Salmond, George P. C. [1 ]
机构
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[2] Natl Inst Biomed Innovat, Osaka 5670085, Japan
[3] Univ Otago, Dept Microbiol & Immunol, Dunedin 9054, New Zealand
基金
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词
PHAGE ABORTIVE INFECTION; CELL-DEATH; SEQUENCE; ALIGNMENTS; DIVERSITY; EVOLUTION; DEFINES; CLONING; SEARCH; FAMILY;
D O I
10.1093/nar/gks231
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Toxin-antitoxin systems are widespread in bacteria and archaea. They perform diverse functional roles, including the generation of persistence, maintenance of genetic loci and resistance to bacteriophages through abortive infection. Toxin-antitoxin systems have been divided into three types, depending on the nature of the interacting macromolecules. The recently discovered Type III toxin-antitoxin systems encode protein toxins that are inhibited by pseudoknots of antitoxic RNA, encoded by short tandem repeats upstream of the toxin gene. Recent studies have identified the range of Type I and Type II systems within current sequence databases. Here, structure-based homology searches were combined with iterative protein sequence comparisons to obtain a current picture of the prevalence of Type III systems. Three independent Type III families were identified, according to toxin sequence similarity. The three families were found to be far more abundant and widespread than previously known, with examples throughout the Firmicutes, Fusobacteria and Proteobacteria. Functional assays confirmed that representatives from all three families act as toxin-antitoxin loci within Escherichia coli and at least two of the families confer resistance to bacteriophages. This study shows that active Type III toxin-antitoxin systems are far more diverse than previously known, and suggests that more remain to be identified.
引用
收藏
页码:6158 / 6173
页数:16
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