Current Methods for Extraction and Concentration of Enteric Viruses from Fresh Fruit and Vegetables: Towards International Standards

被引：44

作者：

Croci, Luciana ^{[2
]}

Dubois, Eric ^{[3
]}

Cook, Nigel ^{[4
]}

de Medici, Dario ^{[2
]}

Schultz, Anna Charlotte ^{[5
]}

China, Bernard ^{[6
]}

Rutjes, Saskia A. ^{[7
]}

Hoorfar, Jeffrey ^{[5
]}

Van der Poel, Wim H. M. ^{[1
]}

机构：

[1] Wageningen Univ Res, Cent Vet Inst, Div Virol, NL-8219 PH Lelystad, Netherlands

[2] Ist Super Sanita, I-00161 Rome, Italy

[3] Agence Francaise Securite Sanit Aliments, F-94706 Maisons Alfort, France

[4] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England

[5] Danish Inst Food & Vet Res, DK-2860 Copenhagen, Denmark

[6] Univ Liege, Fac Vet Med, Dept Food Sci, B-4000 Liege, Belgium

[7] RIVM, Lab Zoonoses & Environm Microbiol, NL-3720 BA Bilthoven, Netherlands

来源：

FOOD ANALYTICAL METHODS | 2008年 / 1卷 / 02期

关键词：

Extraction; Concentration; PCR; Vegetables; Fruit; Norovirus; Hepatitis A Virus;

D O I：

10.1007/s12161-008-9025-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Virus-contaminated soft fruits or vegetables are increasingly identified as causes of foodborne viral illness. Noroviruses and hepatitis A virus are the most common pathogens in viral infections transmitted by these kinds of foods. To improve microbiological detection and monitoring and to increase insights into the contribution of fruits and vegetables to foodborne viral transmission, sensitive, reliable, and standardized methods are needed. More studies on virus detection methods for foods are being published, but validated consensus protocols are not yet available. In this paper, different methodologies are reviewed critically. The use of process controls and internal amplification controls is discussed.

引用

页码：73 / 84

页数：12

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