Simplified avidin-biotin mediated antibody attachment for a surface plasmon resonance biosensor

被引：8

作者：

Anderson, GP ^{[1
]}

Merrick, EC

Trammell, SA

Chinowsky, TM

Shenoy, DK

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA

来源：

SENSOR LETTERS | 2005年 / 3卷 / 02期

关键词：

immunoassay; surface plasmon resonance; biosensor; self-assembled monolayer;

D O I：

10.1166/sl.2005.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The purpose of this research was to develop a reliable and simple method to prepare antibody coated gold surfaces to perform immunoassays on a wavelength interrogated surface plasmon resonance (SPR) biosensor. The gold sensor surface was treated with a commercial disulfide-biotin molecule and NeutrAvidin-a useful biomolecule for both immobilizing biotinylated antibodies and passivating the surface against non-specific binding. This protocol, utilizing simple self assembled monolayers, was both effective and recyclable. The effectiveness of the protocol was tested by performing immunoassays for IgG, Staphylcoccol entertoxin B, ricin, and Bacillus globigii spores.

引用

页码：151 / 156

页数：6

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