Cryo-EM structure of a 3D DNA-origami object

被引：207

作者：

Bai, Xiao-chen ^{[1
]}

Martin, Thomas G. ^{[2
]}

Scheres, Sjors H. W. ^{[1
]}

Dietz, Hendrik ^{[2
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 0QH, England

[2] Tech Univ Munich, Walter Schottky Inst, Dept Phys, D-85748 Garching, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 49期

基金：

英国医学研究理事会; 欧洲研究理事会;

关键词：

HOLLIDAY JUNCTION; CRYSTAL-STRUCTURE; FOLDING DNA; SOFTWARE; SHAPES; MOTOR; TILT;

D O I：

10.1073/pnas.1215713109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.

引用

页码：20012 / 20017

页数：6

共 36 条

[11] High-Resolution Structural Analysis of a DNA Nanostructure by cryoEM [J].