Cholesteryl-silica stationary phase for liquid chromatography - Comparative study of retention behavior and selectivity

被引：79

作者：

Buszewski, B

Jezierska, M

Welniak, M

Kaliszan, R

机构：

[1] Nicholas Copernicus Univ, Dept Environm Chem & Ecoanalyt, Fac Chem, PL-87100 Torun, Poland

[2] Nicholas Copernicus Univ, Dept Organ Chem, Fac Chem, PL-87100 Torun, Poland

[3] Med Univ Gdansk, Dept Biopharmaceut & Pharmacodynam, PL-80416 Gdansk, Poland

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 845卷 / 1-2期

关键词：

stationary phases; LC; retention factors; selectivity; cholesterol-silica stationary phases; polynuclear aromatic hydrocarbons; alkylbenzenes;

D O I：

10.1016/S0021-9673(99)00141-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Retention mechanism, separation possibilities and physicochemical properties of a laboratory-synthesized packing material for high-performance liquid chromatography (HPLC) containing cholesterol moiety immobilized on silica matrix were studied. In order to exhibit the original properties of the new phase, two referential materials - an octadecylsilica and an acylaminopropylsilica phase - were prepared starting from the same silica adsorbent. The chromatographic behavior of the three columns was examined with special regard to retention and selectivity in the reversed-phase HPLC mode employing a variety of mobile phase compositions. Special attention has been paid to possible specific applications of the phases and to temperature effects on retention and selectivity of selected polycyclic aromatic hydrocarbons and other analytes of highly diversified structure. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：433 / 445

页数：13

共 27 条

[1] Determination of solute lipophilicity, as log P(octanol) and log P(alkane) using poly(styrene-divinylbenzene) and immobilised artificial membrane stationary phases in reversed-phase high-performance liquid chromatography [J].