Purification and characterization of a highly enantioselective epoxide hydrolase from Aspergillus niger

被引：58

作者：

Morisseau, C

Archelas, A

Guitton, C

Faucher, D

Furstoss, P

Baratti, JC

机构：

[1] Univ Mediterranee, Fac Sci Luminy, Biocatalysis & Fine Chem Grp, CNRS,ESA 6111, F-13288 Marseille 9, France

[2] Rhone Poulenc Agro, Dept Biotechnol, Lyon, France

[3] Rhone Poulenc Rorer, Gencell Prod & Proc Dev Dept, CRVA, F-94403 Vitry, France

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 263卷 / 02期

关键词：

Aspergillus niger; diols; enantioselectivity; epoxide hydrolase; regioselectivity;

D O I：

10.1046/j.1432-1327.1999.00519.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The epoxide hydrolase from Aspergillus niger was purified to homogeneity using a four-step procedure and p-nitrostyrene oxide (pNSO) as substrate. The enzyme was purified 246-fold with 4% activity yield. The protein is a tetramer composed of four identical subunits of molecular mass 45 kDa. Maximum activity was observed at 40 degrees C, pH 7.0, and with dimethylformamide as cosolvent to dissolve pNSO. Hydrolysis of pNSO was highly enantioselective, with an E value (i.e. enantiomeric ratio) of 40 and a high regioselectivity (97%) for the less hindered carbon atom of the epoxide. This enzyme may be a good biocatalyst for the preparation of enantiopure epoxides or diols.

引用

页码：386 / 395

页数：10

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