Enhancing depth of focus in tilted microfluidics channels by digital holography

被引:15
作者
Matrecano, Marcella [1 ]
Paturzo, Melania
Finizio, Andrea
Ferraro, Pietro
机构
[1] CNR Ist Nazl Ott Applicata, I-80078 Pozzuoli, NA, Italy
关键词
EXTENDED DEPTH; MICROSCOPY; FIELD; IMAGE; SYSTEMS;
D O I
10.1364/OL.38.000896
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
In this Letter we propose a method to enhance the limited depth of field (DOF) in optical imaging systems, through digital holography. The proposed approach is based on the introduction of a cubic phase plate into the diffraction integral, analogous to what occurs in white-light imaging systems. By this approach we show that it is possible to improve the DOF and to recover the extended focus image of a tilted object in a single reconstruction step. Moreover, we demonstrate the possibility of obtaining well-focused biological cells flowing into a tilted microfluidic channel. (C) 2013 Optical Society of America
引用
收藏
页码:896 / 898
页数:3
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