Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications

被引：221

作者：

Cao, Lei ^{[1
,2
]}

Cui, Xingye ^{[1
,2
]}

Hu, Jie ^{[1
,2
]}

Li, Zedong ^{[1
,2
]}

Choi, Jane Ru ^{[1
,2
]}

Yang, Qingzhen ^{[1
,2
]}

Lin, Min ^{[1
,2
]}

Ying HuiLi ^{[3
]}

Xu, Feng ^{[1
,2
]}

机构：

[1] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Minist Educ, Key Lab Biomed Informat Engn, Xian 710049, Peoples R China

[2] Xi An Jiao Tong Univ, BEBC, Xian 710049, Peoples R China

[3] China Astronaut Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Fdn, Beijing 100094, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2017年 / 90卷

基金：

对外科技合作项目（国际科技项目）;

关键词：

Deoxyribonucleic acid; Absolute quantification; Digital PCR; Droplet; Microwell; Microfluidics; Beads; CIRCULATING TUMOR-CELLS; DNA COPY NUMBER; ON-A-CHIP; REAL-TIME; QUANTITATIVE PCR; ISOTHERMAL AMPLIFICATION; ABSOLUTE QUANTIFICATION; DROPLET MICROFLUIDICS; TEMPERATURE-CONTROL; SOFT LITHOGRAPHY;

D O I：

10.1016/j.bios.2016.09.082

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and forensic identification. In the past three decades, PCR has evolved from end-point PCR, through real-time PCR, to its current version, which is the absolute quantitive digital PCR (dPCR). In this review, we first discuss the principles of all key steps of dPCR, i.e., sample dispersion, amplification, and quantification, covering commercialized apparatuses and other devices still under lab development. We highlight the advantages and disadvantages of different technologies based on these steps, and discuss the emerging biomedical applications of dPCR. Finally, we provide a glimpse of the existing challenges and future perspectives for dPCR.

引用

页码：459 / 474

页数：16

共 222 条

[1] Palm-Sized Device for Point-of-Care Ebola Detection [J].

Ahrberg, Christian D. ;

Manz, Andreas ;

Neuzil, Pavel .

ANALYTICAL CHEMISTRY, 2016, 88 (09) :4803-4807

[2] Handheld real-time PCR device [J].

Ahrberg, Christian D. ;

Ilic, Bojan Robert ;

Manz, Andreas ;

Neuzil, Pavel .

LAB ON A CHIP, 2016, 16 (03) :586-592

[3] Role of cell-free plasma DNA as a diagnostic marker for prostate cancer [J].

Allen, D ;

Butt, A ;

Cahill, D ;

Wheeler, M ;

Popert, R ;

Swaminathan, R .

CIRCULATING NUCLEIC ACIDS IN PLASMA/SERUM III AND SERUM PROTEOMICS, 2004, 1022 :76-80

[4] The functions of animal microRNAs [J].

Ambros, V .

NATURE, 2004, 431 (7006) :350-355

[5] Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes [J].

Arrabito, Giuseppe ;

Galati, Clelia ;

Castellano, Sabrina ;

Pignataro, Bruno .

LAB ON A CHIP, 2013, 13 (01) :68-72

[6] Digital PCR hits its stride [J].

Baker, Monya .

NATURE METHODS, 2012, 9 (06) :541-544

[7] Celebrate the unknowns [J].

Ball, Philip .

NATURE, 2013, 496 (7446) :419-420

[8]

Barrett AN, 2014, METHODS MOL BIOL, V1160, P215, DOI 10.1007/978-1-4939-0733-5_17

[9] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[10]

Bartlett John M S, 2003, Methods Mol Biol, V226, P3

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