Over-expressed microRNA-27a and 27b influence fat accumulation and cell proliferation during rat hepatic stellate cell activation

被引：416

作者：

Ji, Juling ^{[1
,2
]}

Zhang, Jinsheng ^{[1
]}

Huang, Guangcun ^{[1
]}

Qian, Jin ^{[1
]}

Wang, Xueqing ^{[1
]}

Mei, Shuang ^{[1
]}

机构：

[1] Fudan Univ, Dept Pathol, Shanghai Med Coll, Shanghai 200032, Peoples R China

[2] Nantong Univ, Dept Pathol, Coll Med, Nantong 226001, Peoples R China

来源：

FEBS LETTERS | 2009年 / 583卷 / 04期

关键词：

MicroRNA-27; Hepatic stellate cell; Fat accumulation; Cell proliferation; Retinoid X receptor alpha; Liver fibrosis; CYCLE REGULATION; CARCINOMA-CELLS; IN-VITRO; DIFFERENTIATION; TARGETS; SPECIFICITY; PHENOTYPE; RETINOIDS; RNA;

D O I：

10.1016/j.febslet.2009.01.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Hepatic stellate cells (HSCs) activation is an initial event in liver. fibrosis. MicroRNAs (miRNAs) have been found to play essential roles in cell differentiation, proliferation, and fat metabolism. In this study, we showed that down-regulation of two over-expressed miRNAs, miR-27a and 27b allowed culture-activated rat HSCs to switch to a more quiescent HSC phenotype, with restored cytoplasmic lipid droplets and decreased cell proliferation. Mechanistically, retinoid X receptor alpha was confirmed to be the target of miR-27a and 27b. These results indicated a new role and mechanism of miR-27a and 27b in regulating fat metabolism and cell proliferation during HSCs activation. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

收藏

页码：759 / 766

页数：8

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