Molecular cloning of synaphins/complexins, cytosolic proteins involved in transmitter release, in the electric organ of an electric ray (Narke japonica)

被引：11

作者：

Ishizuka, T

Saisu, H

Suzuki, T

Kirino, Y

Abe, T

机构：

[1] NIIGATA UNIV,BRAIN RES INST,DEPT CELLULAR NEUROBIOL,NIIGATA 951,JAPAN

[2] UNIV TOKYO,SCH PHARMACEUT SCI,LAB NEUROBIOPHYS,TOKYO 113,JAPAN

来源：

NEUROSCIENCE LETTERS | 1997年 / 232卷 / 02期

关键词：

synaphin/complexin; transmitter release; docking/fusion complex; Narke japonica; electric organ; syntaxin; SNAP-25; VAMP/synaptobrevin;

D O I：

10.1016/S0304-3940(97)00586-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaphins/complexins are cytosolic proteins associated with the docking/fusion complex crucial to transmitter release, The electric organ of the electric ray Narke japonica contained at least two kinds of synaphins as revealed by immunoblotting. cDNAs for three synaphins were cloned from a cDNA library prepared from the electric lobe where cell bodies of electromotor nerves innervating the electric organ exist. The proteins encoded by these cDNAs were named Nj-synaphins 1a, 1b and 2 on the basis of their high homologies (83-93%) to mammalian synaphins 1 and 2. Nj-Synaphins were immunoprecipitated by an anti-syntaxin monoclonal antibody, together with syntaxin, SNAP-25 and VAMP (synaptobrevin), suggesting the presence of a docking/fusion complex similar to that in the mammalian brain. (C) 1997 Elsevier Science Ireland Ltd.

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页码：107 / 110

页数：4

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