Strong electromagnetic confinement near dielectric microspheres to enhance single-molecule fluorescence

被引：98

作者：

Gerard, Davy ^{[1
]}

Wenger, Jerome ^{[1
]}

Devilez, Alexis ^{[1
]}

Gachet, David ^{[1
]}

Stout, Brian ^{[1
]}

Bonod, Nicolas ^{[1
]}

Popov, Evgeny ^{[1
]}

Rigneault, Herve ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, Inst Fresnel, F-13397 Marseille, France

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 19期

关键词：

D O I：

10.1364/OE.16.015297

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Latex microspheres are used as a simple and low-cost means to achieve three axis electromagnetic confinement below the standard diffraction limit. We demonstrate their use to enhance the fluorescence fluctuation detection of single molecules. Compared to confocal microscopy with high numerical aperture, we monitor a detection volume reduction of one order of magnitude below the diffraction limit together with a 5-fold gain in the fluorescence rate per molecule. This offers new opportunities for a broad range of applications in biophotonics, plasmonics, optical data storage and ultramicroscopy. (c) 2008 Optical Society of America.

引用

页码：15297 / 15303

页数：7

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