Methods for studying global patterns of DNA binding by bacterial transcription factors and RNA polymerase

被引：11

作者：

Grainger, David C. ^{[1
]}

Busby, Stephen J. W. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2008年 / 36卷

关键词：

chromatin immunoprecipitation (ChIP); DNA binding; Escherichia coli; microarray; transcription;

D O I：

10.1042/BST0360754

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A major goal in the study of gene regulation is to untangle the transcription-regulatory networks of Escherichia coli and other 'simple' organisms. To do this we must catalogue the binding sites of all transcription factors. ChIP (chromatin immunoprecipitation), combined with DNA microarray analysis, is a powerful tool that permits global patterns of DNA binding to be measured. Here, we discuss the benefits of this approach and the application of this technique to bacterial systems.

引用

页码：754 / 757

页数：4

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