Harmonic imaging with levovist

Our purpose was to test the hypothesis that second harmonic imaging preferentially detects backscatter from microbubbles compared with tissue structural components. A prototype second harmonic scanner was used to image a flow channel in a tissue-mimicking rubber phantom (liver density). Video time-intensity curves were calculated from repeated bolus injections of microbubble echocardiographic contrast material under the same fluid dynamic conditions but with three different imaging modes: (1) fundamental imaging at 2.5 MHz (transmit and receive at 2.5 MHz), (2) fundamental imaging at 5.0 MHz (transmit and receive at 5.0 MHz), and (3) second harmonic imaging (transmit at 2.5 MHz and receive at 5.0 MHz). Each video time-intensity curve was calibrated such that quantitative backscatter intensity was measured relative to the tissue phantom (0 dB). The peak increase in backscatter from the contrast material in the channel relative to the tissue phantom and the intensity in the channel before the contrast effect (the noise floor) was measured along with the area under the calibrated time-intensity curve relative to the phantom. When referenced to the noise floor in the how channel, all imaging modes produced approximately 25 dB of enhancement. However, when referenced to the tissue phantom, second harmonic imaging produced a 22.3 +/- 1.8 dB peak enhancement, which was greater than either fundamental imaging at 2.5 MHz (15.5 +/- 0.8 dB; p < 0.001) or fundamental imaging at 5.0 MHz (15.3 +/- 1.5 dB; p < 0.001). The area under the time-intensity curves confirmed that harmonic imaging has approximately 7 dB of relative enhancement to the phantom compared with fundamental imaging at either frequency. Second harmonic imaging specifically enhances backscatter from microbubbles compared with a tissue-mimicking phantom. This specificity for microbubbles is due to a decrease in backscatter for the tissue phantom, rather than an increase in backscatter for the microbubbles. These data support the hypothesis that second harmonic imaging may be able to detect microbubbles in the tissue vascular space by preferentially decreasing the backscatter from tissue structural components.