A combination of techniques to evaluate photodynamic efficiency of photosensitizers

被引:60
作者
Cavalcante, R. S. [1 ]
Imasato, H. [1 ]
Bagnato, V. S. [2 ]
Perussi, J. R. [1 ]
机构
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560590 Sao Carlos, SP, Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560590 Sao Carlos, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
photodynamic therapy; hematoporphyrin derivatives; chlorin derivative; photodynamic efficiency; photo-hemolysis; albumin; PARTITION-COEFFICIENTS; SINGLET OXYGEN; PHOTOGEM(R); THERAPY; HYDROPHOBICITY; PHOTOSTABILITY; PHOTOPRODUCTS; CYTOTOXICITY; PHOTOFRIN(R); AGGREGATION;
D O I
10.1002/lapl.200810082
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Photodynamic therapy, used mainly for cancer treatment and microorganisms inaction, is based on production of reactive oxygen species by light irradiation of a sensitizer. Hematoporphyrin derivatives as Photofrin (R) (PF) Photogem (R) (PG) and Photosan (R) (PF), and chlorin-c6-derivatives as Photodithazine (R)(PZ), have suitable sensitizing properties. The present study provides a way to make a fast previous evaluation of photosensitizers efficacy by a combination of techniques: a) use of brovine serum albumin and uric acid as chemical dosimeters; b) photo-hemolysis of red blood cells used as a cell membrane interaction model, and c) octanol/phosphate buffer partition to assess the relative lipophilicity of the compounds. The results suggest the photodynamic efficient rankings PZ > PG >= PF > PS. These results agree with the cytotoxicity of the photosensitizers as well as to chromatographic separation of the HpDs, both performed in our group, showing that the more lipophilic is the dye, the more acute is the damage to the RBC membrane and the oxidation of indol, which is immersed in the hydrophobic region of albumin.
引用
收藏
页码:64 / 70
页数:7
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