It has often been assumed rotational kinematics are improved with mobile-bearing TKA designs as the terms mobile-bearing and rotating platform imply. We tested this assumption by assessing the in vivo axial rotation magnitudes and patterns of 527 knees implanted with 12 different mobile-bearing TKA designs. Implants were grouped and compared by type-posterior stabilized (PS), posterior cruciate retaining (PCR), and posterior cruciate sacrificing (PCS)-and by specific design. We hypothesized all three mobile-bearing types (PS, PCR, and PCS) would achieve greater than 10 degrees average axial rotation and we would find no differences in axial rotation between types. Only 14% of PS knees, 3% of PCS knees, and 17% of PCR knees attained greater than 10 degrees axial rotation when measured from 0 degrees to 90 degrees. The percentage of PCS knees with greater than 10 degrees axial rotation was less compared with the other two groups. Axial rotation averaged 4.3 degrees, 2.5 degrees, and 3.8 degrees for the PS, PCS, and PCR knees, respectively. Incidences of reverse rotation were observed in 17% of PS knees, 32% of PCS knees, and 28% of PCR knees. Compared with the PCS group, the PS group achieved greater average axial rotation and had a lower percentage of knees displaying incidences of reverse rotation. The data refuted the hypotheses.