Rapid fractionation of grape seed proanthocyanidins

被引：79

作者：

Saucier, C ^{[1
]}

Mirabel, M ^{[1
]}

Daviaud, F ^{[1
]}

Longieras, A ^{[1
]}

Glories, Y ^{[1
]}

机构：

[1] Fac Enol, Lab Chim Appl, F-33405 Talence, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 12期

关键词：

grape; seed; proanthocyanidin; size; molecular weight; fractionation;

D O I：

10.1021/jf010784f

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A rapid method that permits separation of grape seed proanthocyanidins according to their polymerization degrees has been developed. This method was based on liquid/liquid extraction and relative solubility of these compounds in different solvents (water, ethyle acetate, methanol, and chloroform). The different fractions obtained were then analyzed by various HPLC techniques (normal phase, reversed phase after thiolysis, and gel permeation) to determine their mean degree of polymerization (DP) and molecular weight profiles. Results show that this method can be used on a preparative scale to separate these polymers according to their sizes.

引用

收藏

页码：5732 / 5735

页数：4

相关论文

共 25 条

[11] POLYPHENOL-PROTEIN INTERACTIONS [J].

HASLAM, E .

BIOCHEMICAL JOURNAL, 1974, 139 (01) :285-288

[12] Changes in grape seed polyphenols during fruit ripening [J].

Kennedy, JA ;

Matthews, MA ;

Waterhouse, AL .

PHYTOCHEMISTRY, 2000, 55 (01) :77-85

[13] Quantitative fractionation of grape proanthocyanidins according to their degree of polymerization [J].

Labarbe, B ;

Cheynier, V ;

Brossaud, F ;

Souquet, JM ;

Moutounet, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (07) :2719-2723

[14] PHENOLICS OF CIDERS - BITTERNESS AND ASTRINGENCY [J].

LEA, AGH ;

ARNOLD, GM .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1978, 29 (05) :478-483

[15] Method for estimation of proanthocyanidins based on their acid depolymerization in the presence of nucleophiles [J].

Matthews, S ;

Mila, I ;

Scalbert, A ;

Pollet, B ;

Lapierre, C ;

duPenhoat, CLMH ;

Rolando, C ;

Donnelly, DMX .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (04) :1195-1201

[16] HEMANALYSIS - THE RELATIVE ASTRINGENCY OF PROANTHOCYANIDIN POLYMERS [J].

PORTER, LJ ;

WOODRUFFE, J .

PHYTOCHEMISTRY, 1984, 23 (06) :1255-1256

[17] OLIGOMERIC AND POLYMERIC PROCYANIDINS FROM GRAPE SEEDS [J].

PRIEUR, C ;

RIGAUD, J ;

CHEYNIER, V ;

MOUTOUNET, M .

PHYTOCHEMISTRY, 1994, 36 (03) :781-784

[18] PROCYANIDIN DIMERS AND TRIMERS FROM GRAPE SEEDS [J].

RICARDO-DA-SILVA, JM ;

RIGAUD, J ;

CHEYNIER, V ;

CHEMINAT, A ;

MOUTOUNET, M .

PHYTOCHEMISTRY, 1991, 30 (04) :1259-1264

[19] MICRO METHOD FOR THE IDENTIFICATION OF PROANTHOCYANIDIN USING THIOLYSIS MONITORED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

RIGAUD, J ;

PEREZILZARBE, J ;

RICARDO-DA-SILVA, JM ;

CHEYNIER, V .

JOURNAL OF CHROMATOGRAPHY, 1991, 540 (1-2) :401-405

[20] NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PROCYANIDINS FROM CACAO BEANS AND GRAPE SEEDS [J].

RIGAUD, J ;

ESCRIBANOBAILON, MT ;

PRIEUR, C ;

SOUQUET, JM ;

CHEYNIER, V .

JOURNAL OF CHROMATOGRAPHY A, 1993, 654 (02) :255-260