Holographically encoded microparticles for bead-based assays

被引:4
作者
Birtwell, Sam W. [1 ,3 ]
Banu, Shahanara [1 ]
Zheludev, Nikolay I. [2 ]
Morgan, Hywel [1 ]
机构
[1] Univ Southampton, Sch Elect & Comp Sci, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Optoelect Res Ctr, Southampton SO17 1BJ, Hants, England
[3] Univ Southampton, Sch Phys & Astron, Southampton SO17 1BJ, Hants, England
基金
英国工程与自然科学研究理事会;
关键词
IRREVERSIBLE PHOTOCHROMIC SYSTEMS; DATA-STORAGE; QUANTUM DOTS; ARCHIP EPOXY; SU-8; IMMOBILIZATION; MICROCARRIERS; LITHOGRAPHY; BARCODES; ARRAYS;
D O I
10.1088/0022-3727/42/5/055507
中图分类号
O59 [应用物理学];
学科分类号
摘要
We demonstrate a re-writable, high capacity holographic encoding technique for multiplexed bead-based suspension assays. The microparticles are made from SU8 doped with a photochromic diarylethene dye and manufactured using multilayer photolithography and dry etching. Each particle is encoded with a unique hologram, whose diffraction pattern consists of bright and dark regions, representing a binary number that identifies the particle. Theoretically up to 10(24) unique codes are available on a 100 mu m particle using this method, when the code is read with a standard 2/3" CMOS camera. Encoding capacities of 512 unique codes have been demonstrated on a 500 mu m SU8 particle. The code is thermally stable for 3 days at 25 degrees C, and once written, the code can be erased and re-written once whilst still remaining readable. The code can be written into the particle during an assay experiment (no pre-encoding is required) and requires simple optics for reading.
引用
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页数:7
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