Molecular Imaging Using X-Ray Free-Electron Lasers

被引：147

作者：

Barty, Anton ^{[1
]}

Kuepper, Jochen ^{[1
,2
]}

Chapman, Henry N. ^{[1
,2
]}

机构：

[1] DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[2] Univ Hamburg, Dept Phys, D-22761 Hamburg, Germany

来源：

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64 | 2013年 / 64卷

关键词：

coherent imaging; molecular dynamics; structural biology; image orientation; PHASE-RETRIEVAL; MICROSCOPY; RESOLUTION; RADIATION; CRYSTALLOGRAPHY; SYMMETRIES; OPERATION; OBJECTS; SPIDER;

D O I：

10.1146/annurev-physchem-032511-143708

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The opening of hard X-ray free-electron laser facilities, such as the Linac Coherent Light Source (LCLS) at SLAC National Accelerator Laboratory in the United States, has ushered in a new era in structural determination. With X-ray pulse durations down to 10 fs or shorter, and up to 10(13) transversely coherent photons per pulse in a narrow spectral bandwidth, focused irradiances of 10(18) to 10(21) W cm(-2) or higher can be produced at X-ray energies ranging from 500 eV to 10 keV. New techniques for determining the structure of systems that cannot be crystallized and for studying the time-resolved behavior of irreversible reactions at femtosecond timescales are now available.

引用

页码：415 / 435

页数：21

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