Microarray analysis of differentiation-specific gene expression during 3T3-L1 adipogenesis

被引:78
作者
Burton, GR
Nagarajan, R
Peterson, CA
McGehee, RE
机构
[1] Univ Arkansas Med Sci, Dept Pediat, Div Neonatol, Little Rock, AR 72205 USA
[2] Donald W Reynolds Ctr Aging, Dept Geriatr, Little Rock, AR 72205 USA
[3] Arkansas Canc Res Ctr, Little Rock, AR 72205 USA
[4] Cent Arkansas Vet Hlth Care Syst, Little Rock, AR 72205 USA
[5] Arkansas Childrens Hosp, Little Rock, AR 72205 USA
关键词
3T3-L1; cells; adipogenesis; cDNA/EST; adipocyte; hierarchical clustering; self-organizing maps;
D O I
10.1016/j.gene.2003.12.012
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
During cellular differentiation and development, it is recognized that many complex molecular mechanisms as well as precise patterns of differentially expressed genes occur in directing precursor cells toward a given lineage. Using microarray-based technology, we examined gene expression across the course of 3T3-L1 adipocyte differentiation. Total cellular RNA was isolated at times 0, 2, 8, 16, 24, 48, and 96 h following treatment with either standard hormonal inducers of differentiation; insulin, dexamethasone, isobutylmethylxanthine (IDX), or IDX plus trichostatin A (TsA), a historic deacetylase inhibitor and potent adipogenic inhibitor. cRNA was synthesized from cellular RNA and hybridized to high density Affymetrix MG_U74Av2 microarray gene chips containing 12,488 cDNA/Expressed Sequence Tags (ESTs) probe sets. From the IDX-only treated cells, all probe sets that were either unchanged or differentially expressed less than 2-fold throughout differentiation with respect to time 0 preadipocytes were excluded from further analyses. This selection resulted in a net of 1686 transcripts, 859 were increased in expression, and 827 were decreased in expression at least 2-fold across differentiation. To focus in on genes that were more specific to differentiation, the same analysis was performed on IDX plus TsA-treated non-differentiating cells and all probe sets from the IDX-only group that exhibited similar expression profiles in the non-differentiating TsA-treated group were excluded leaving a total of 1016 transcripts that were regulated only under differentiating conditions. Six hundred and thirty-six of these transcripts were elevated at least 2-fold and 380 exhibited a decrease in expression relative to time 0 preadipocytes. This group of genes was further analyzed using hierarchical clustering and self-organizing maps and resulted in the identification of numerous genes not previously known to be regulated during adipocyte differentiation. Many of these genes may well represent novel adipogenic mediators and markers of adipogenesis. (C) 2004 Elsevier B.V All rights reserved.
引用
收藏
页码:167 / 185
页数:19
相关论文
共 34 条
  • [11] 43::AID-DVG5&gt
  • [12] 3.0.CO
  • [13] 2-7
  • [14] Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation
    Kobayashi, K
    Hatano, M
    Otaki, M
    Ogasawara, T
    Tokuhisa, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) : 1457 - 1462
  • [15] Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection
    Li, C
    Wong, WH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (01) : 31 - 36
  • [16] CCAAT/ENHANCER BINDING-PROTEIN-ALPHA IS SUFFICIENT TO INITIATE THE 3T3-L1 ADIPOCYTE DIFFERENTIATION PROGRAM
    LIN, FT
    LANE, MD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (19) : 8757 - 8761
  • [17] ANTISENSE CCAAT ENHANCER-BINDING PROTEIN RNA SUPPRESSES COORDINATE GENE-EXPRESSION AND TRIGLYCERIDE ACCUMULATION DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES
    LIN, FT
    LANE, MD
    [J]. GENES & DEVELOPMENT, 1992, 6 (04) : 533 - 544
  • [18] Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor
    Liu, Q
    Yu, L
    Gao, J
    Fu, Q
    Zhang, JX
    Zhang, PZ
    Chen, J
    Zhao, SY
    [J]. MOLECULAR BIOLOGY REPORTS, 2000, 27 (04) : 241 - 246
  • [19] Expression monitoring by hybridization to high-density oligonucleotide arrays
    Lockhart, DJ
    Dong, HL
    Byrne, MC
    Follettie, MT
    Gallo, MV
    Chee, MS
    Mittmann, M
    Wang, CW
    Kobayashi, M
    Horton, H
    Brown, EL
    [J]. NATURE BIOTECHNOLOGY, 1996, 14 (13) : 1675 - 1680
  • [20] TNFα disrupts mitotic clonal expansion and regulation of retinoblastoma proteins p130 and p107 during 3T3-L1 adipocyte differentiation
    Lyle, RE
    Richon, VM
    McGehee, RE
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 247 (02) : 373 - 378