Solid-phase extraction and liquid chromatography-electrospray mass spectrometric analysis of saponins in a Chinese patent medicine of formulated Salvia miltiorrhizae and Panax notoginseng

被引：39

作者：

Zhang, HJ ^{[1
]}

Cheng, YY ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310027, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 40卷 / 02期

关键词：

Panax notoginseng; Salvia miltiorrhizae; saponin; solid-phase extraction; HPLC/ESI-MSn;

D O I：

10.1016/j.jpba.2005.07.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, a method using solid-phase extraction (SPE) and HPLC/ESI-MS" for the identification of the major saponins in "Danshen Dripping Pill", a Chinese patent medicine consisting of Salvia miltiorrhizae and Panax notoginseng, is described. Through solid-phase extraction process, the saponins in "Danshen Dripping Pill" were separated from the phenolic constituents of S. miltiorrhizae and, meanwhile, efficiently concentrated. Subsequently, these saponins were characterized by HPLC/ESI-MS" analysis. Based on the studies of MS and MS2 spectra and the comparison with reference compounds and literature data, a total of 19 saponins were identified. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：429 / 432

页数：4

共 11 条

[1] Ginseng pharmacology - Multiple constituents and multiple actions [J].

Attele, AS ;

Wu, JA ;

Yuan, CS .

BIOCHEMICAL PHARMACOLOGY, 1999, 58 (11) :1685-1693

[2]

Cui M, 2000, RAPID COMMUN MASS SP, V14, P1280, DOI 10.1002/1097-0231(20000730)14:14<1280::AID-RCM26>3.0.CO

[3]

2-C

[4] Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots [J].

Fuzzati, N ;

Gabetta, B ;

Jayakar, K ;

Pace, R ;

Peterlongo, F .

JOURNAL OF CHROMATOGRAPHY A, 1999, 854 (1-2) :69-79

[5]

Huang Y.Z., 2002, TRAD CHIN DRUG RES C, V13, P174

[6] Liquid chromatographic determination of less polar ginsenosides in processed ginseng [J].

Kwon, SW ;

Han, SB ;

Park, IH ;

Kim, JM ;

Park, MK ;

Park, JH .

JOURNAL OF CHROMATOGRAPHY A, 2001, 921 (02) :335-339

[7] Liquid chromatographic method for determination of four active saponins from Panax notoginseng in rat urine using solid-phase extraction [J].

Li, L ;

Zhang, JL ;

Sheng, YX ;

Ye, G ;

Guo, HZ ;

Guo, DA .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 808 (02) :177-183

[8] Study of the determination and pharmacokinetics of Compound Danshen Dripping Pills in human serum by column switching liquid chromatography electrospray ion trap mass spectrometry [J].

Pei, WJ ;

Zhao, XF ;

Zhu, ZM ;

Lin, CZ ;

Zhao, WM ;

Zheng, XH .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 809 (02) :237-242

[9]

*STAT PHARM COMM P, 2000, PHARM PR CHIN, V1

[10] Analysis of 'SHENMAI' injection by HPLC/MS/MS [J].

Zhang, HJ ;

Wu, YJ ;

Cheng, YY .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 31 (01) :175-183

← 1 2 →