Physical-chemical plant-derived signals induce differentiation in Ustilago maydis

被引:99
作者
Mendoza-Mendoza, Artemio [1 ]
Berndt, Patrick [1 ]
Djamei, Armin [1 ]
Weise, Carolin [1 ]
Linne, Uwe [2 ]
Marahiel, Mohamed [2 ]
Vranes, Miroslav [1 ]
Kaemper, Joerg [1 ]
Kahmann, Regine [1 ]
机构
[1] Max Planck Inst Terr Microbiol, Dept Organism Interact, D-35043 Marburg, Germany
[2] Univ Marburg, Dept Biochem, D-35032 Marburg, Germany
关键词
RICE BLAST FUNGUS; MEDIATES APPRESSORIUM DIFFERENTIATION; FILAMENTOUS GROWTH; MAGNAPORTHE-GRISEA; PATHOGENIC DEVELOPMENT; INFECTION; PENETRATION; PROTEIN; CAMP; GENE;
D O I
10.1111/j.1365-2958.2008.06567.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ustilago maydis is able to initiate pathogenic development after fusion of two haploid cells with different mating type. On the maize leaf surface, the resulting dikaryon switches to filamentous growth, differentiates appressoria and penetrates the host. Here, we report on the plant signals required for filament formation and appressorium development in U. maydis. In vitro, hydroxy-fatty acids stimulate filament formation via the induction of pheromone genes and this signal can be bypassed by genetically activating the downstream MAP kinase module. Hydrophobicity also induces filaments and these resemble the dikaryotic filaments formed on the plant surface. With the help of a marker gene that is specifically expressed in the tip cell of those hyphae that have formed an appressorium, hydrophobicity is shown to be essential for appressorium development in vitro. Hydroxy-fatty acids or a cutin monomer mixture isolated from maize leaves have a stimulatory role when a hydrophobic surface is provided. Our results suggest that the early phase of communication between U. maydis and its host plant is governed by two different stimuli.
引用
收藏
页码:895 / 911
页数:17
相关论文
共 70 条
[41]   Formation of plant cuticle:: evidence for the occurrence of the peroxygenase pathway [J].
Lequeu, J ;
Fauconnier, ML ;
Chammaï, A ;
Bronner, R ;
Blée, E .
PLANT JOURNAL, 2003, 36 (02) :155-164
[42]   BEHAVIOR OF UREDOSPORE GERM-TUBES OF PUCCINIA-GRAMINIS-TRITICI IN RELATION TO FINE-STRUCTURE OF WHEAT LEAF SURFACES [J].
LEWIS, BG ;
DAY, JR .
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1972, 58 (FEB) :137-&
[43]   Rgs1 regulates multiple Gα subunits in Magnaporthe pathogenesis, asexual growth and thigmotropism [J].
Liu, Hao ;
Suresh, Angayarkanni ;
Willard, Francis S. ;
Siderovski, David P. ;
Lu, Shen ;
Naqvi, Naweed I. .
EMBO JOURNAL, 2007, 26 (03) :690-700
[44]   A homologue of the transcriptional repressor Ssn6p antagonizes cAMP signalling in Ustilago maydis [J].
Loubradou, G ;
Brachmann, A ;
Feldbrügge, M ;
Kahmann, R .
MOLECULAR MICROBIOLOGY, 2001, 40 (03) :719-730
[45]   A MAP kinase encoded by the ubc3 gene of Ustilago maydis is required for filamentous growth and full virulence [J].
Mayorga, ME ;
Gold, SE .
MOLECULAR MICROBIOLOGY, 1999, 34 (03) :485-497
[46]   TANSLEY REVIEW NO 48 INFECTION STRUCTURES OF FUNGAL PLANT-PATHOGENS - A CYTOLOGICAL AND PHYSIOLOGICAL EVALUATION [J].
MENDGEN, K ;
DEISING, H .
NEW PHYTOLOGIST, 1993, 124 (02) :193-213
[47]   An Ustilago maydis gene involved in H2O2 detoxification is required for virulence [J].
Molina, Lazaro ;
Kahmann, Regine .
PLANT CELL, 2007, 19 (07) :2293-2309
[48]   Mating and pathogenic development of the smut fungus Ustilago maydis are regulated by one mitogen-activated protein kinase cascade [J].
Müller, P ;
Weinzierl, G ;
Brachmann, A ;
Feldbrügge, M ;
Kahmann, R .
EUKARYOTIC CELL, 2003, 2 (06) :1187-1199
[49]   Preparation, isolation, and characterization of cutin monomers and oligomers from tomato peels [J].
Osman, SF ;
Irwin, P ;
Fett, WF ;
O'Connor, JV ;
Parris, N .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (02) :799-802
[50]  
OTTO K, 2002, P NATL ACAD SCI USA, V92, P4080