Rapid HPLC-ESI-MS/MS for qualitative and quantitative analysis of saponins in "XUESETONG" injection

被引：48

作者：

Lai, CM ^{[1
]}

Li, SP ^{[1
]}

Yu, H ^{[1
]}

Wan, JB ^{[1
]}

Kan, KW ^{[1
]}

Wang, YT ^{[1
]}

机构：

[1] Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 40卷 / 03期

关键词：

XUESETONG injection; Panax notoginseng; total saponins; HPLC-ESI-MS/MS; quality control; notoginsenoside; ginsenoside;

D O I：

10.1016/j.jpba.2005.11.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

'XUESETONG' injection, one of the most widely used proprietary medicines in traditional Chinese medicine, consists of total saponins made from Panax notoginseng, which is a highly valued and important Chinese medicinal herb. It is used to treat cardiovascular diseases. In order to control the quality of XUESETONG injection, a rapid HPLC-ESI-MS/MS method was developed for qualitative and quantitative determination of the saponins. The analyses were performed on SB-C18 column using gradient elution in 25 min. Full scan and time programmed selected reaction monitoring (SRM) were used for qualitative and quantitative analysis of saponins, respectively. Twenty-seven saponins were identified and nine of them including notoginsenoside R1, ginsenoside Rb1, Rb2, Rb3, Rc, Rd, Re, Rf and Rg1 were quantified. Ten XUESETONG injections were analyzed and compared. The results showed that there is a great variation among different samples. In conclusion, the developed method is rapid, accurate and sensitive for qualitative and quantitative analysis of saponins in XUESETONG injection. Moreover, it also can be used for the quality control of P notoginseng raw material and its preparations. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：669 / 678

页数：10

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