Toxic waste disposal in Escherichia coli

被引：65

作者：

Helling, RB ^{[1
]}

Janes, BK ^{[1
]}

Kimball, H ^{[1
]}

Tran, T ^{[1
]}

Bundesmann, M ^{[1
]}

Check, P ^{[1
]}

Phelan, D ^{[1
]}

Miller, C ^{[1
]}

机构：

[1] Univ Michigan, Dept Biol, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 13期

关键词：

D O I：

10.1128/JB.184.13.3699-3703.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

About 10% of the nalidixic acid-resistant (Nal(r)) mutants in a transposition-induced library exhibited a growth factor requirement as the result of cysH, icdA, metE, or purB mutation. Resistance in all of these mutants required a functional AcrAB-ToIC efflux pump, but the EmrAB-ToIC pump played no obvious role. Transcription of acrAB was increased in each type of Nal(r) mutant. In the icdA and purB mutants, each or the known signaling pathways appeared to be used in activating the AcrAB-ToIC pump. The metabolites that accumulate upstream of the blocks caused by the mutations are hypothesized to increase the levels of the AcrAB-ToIC pump, thereby removing nalidixic acid from the organism.

引用

收藏

页码：3699 / 3703

页数：5

相关论文

共 40 条

[1] The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3 Å resolution [J].

Alekshun, MN ;

Levy, SB ;

Mealy, TR ;

Seaton, BA ;

Head, JF .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (08) :710-714

[2] Alteration of the repressor activity of MarR, the negative regulator of the Escherichia coli marRAB locus, by multiple chemicals in vitro [J].

Alekshun, MN ;

Levy, SB .

JOURNAL OF BACTERIOLOGY, 1999, 181 (15) :4669-4672

[3] The mar regulon:: multiple resistance to antibiotics and other toxic chemicals [J].

Alekshun, MN ;

Levy, SB .

TRENDS IN MICROBIOLOGY, 1999, 7 (10) :410-413

[4] Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12 [J].

Aono, R ;

Tsukagoshi, N ;

Yamamoto, M .

JOURNAL OF BACTERIOLOGY, 1998, 180 (04) :938-944

[5] ACTIVATION OF MULTIPLE ANTIBIOTIC-RESISTANCE AND BINDING OF STRESS-INDUCIBLE PROMOTERS BY ESCHERICHIA-COLI ROB PROTEIN [J].

ARIZA, RR ;

LI, ZY ;

RINGSTAD, N ;

DEMPLE, B .

JOURNAL OF BACTERIOLOGY, 1995, 177 (07) :1655-1661

[6]

BERG DE, 1980, COLD SPRING HARB SYM, V45, P115

[7] SALICYLATE INDUCTION OF ANTIBIOTIC-RESISTANCE IN ESCHERICHIA-COLI - ACTIVATION OF THE MAR OPERON AND A MAR-INDEPENDENT PATHWAY [J].

COHEN, SP ;

LEVY, SB ;

FOULDS, J ;

ROSNER, JL .

JOURNAL OF BACTERIOLOGY, 1993, 175 (24) :7856-7862

[8] MUTANTS OF ESCHERICHIA-COLI REQUIRING METHIONINE OR VITAMIN-B12 [J].

DAVIS, BD ;

MINGIOLI, ES .

JOURNAL OF BACTERIOLOGY, 1950, 60 (01) :17-28

[9] Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli [J].

Fralick, JA .

JOURNAL OF BACTERIOLOGY, 1996, 178 (19) :5803-5805

[10] NALIDIXIC-ACID RESISTANCE - 2ND GENETIC CHARACTER INVOLVED IN DNA GYRASE ACTIVITY [J].

GELLERT, M ;

MIZUUCHI, K ;

ODEA, MH ;

ITOH, T ;

TOMIZAWA, JI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (11) :4772-4776

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