Structures of the human and Drosophila 80S ribosome

被引：440

作者：

Anger, Andreas M. ^{[1
,2
]}

Armache, Jean-Paul ^{[1
,2
]}

Berninghausen, Otto ^{[1
,2
]}

Habeck, Michael ^{[3
,4
]}

Subklewe, Marion ^{[5
,6
]}

Wilson, Daniel N. ^{[1
,2
]}

Beckmann, Roland ^{[1
,2
]}

机构：

[1] Univ Munich, Dept Biochem, Gene Ctr, D-81377 Munich, Germany

[2] Univ Munich, Ctr Integrated Prot Sci Munich CiPSM, D-81377 Munich, Germany

[3] Max Planck Inst Biol Cybernet, Dept Empir Inference, D-72076 Tubingen, Germany

[4] Max Planck Inst Dev Biol, Dept Prot Evolut, D-72076 Tubingen, Germany

[5] Klinikum Univ Munchen, Dept Internal Med 3, D-81377 Munich, Germany

[6] Helmholtz Inst Munich, Clin Cooperat Grp Immunotherapy, D-81377 Munich, Germany

来源：

NATURE | 2013年 / 497卷 / 7447期

基金：

欧洲研究理事会;

关键词：

SECONDARY STRUCTURE; ELECTRON-MICROSCOPY; ATOMIC STRUCTURES; CRYSTAL-STRUCTURE; MESSENGER-RNA; SEQUENCE; SUBUNIT; INITIATION; VISUALIZATION; TRANSLATION;

D O I：

10.1038/nature12104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.

引用

页码：80 / +

页数：7

共 72 条

[31] A Blind Deconvolution Approach for Improving the Resolution of Cryo-EM Density Maps [J].