Quantification of 5′-monophosphate cytosine arabinoside (Ara-CMP) in cell extracts using liquid chromatography-electrospray mass spectrometry

被引：9

作者：

Gouy, Marie-Helene

Fabre, Huguette

Blanchin, Marie Dominique

Peyrottes, Suzanne

Perigaud, Christian

Lefebvre, Isabelle

机构：

[1] Univ Montpellier 1, Chim Analyt Lab, UMR 5625, Fac Pharm, F-34093 Montpellier 05, France

[2] Univ Montpellier 2, UMR 5625, CNRS, Lab Chim Organ Biomol Synth, F-34095 Montpellier 05, France

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 566卷 / 02期

关键词：

Ara-CMP; quantification; liquid chromatography-mass spectrometry; cell extracts;

D O I：

10.1016/j.aca.2006.02.058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A LC/MS/ESI method is described to monitor 5 '-monophosphate cytosine arabinoside (Ara-CMP) formed after incubation of an Ara-C prodrug in cell extracts. After addition of an internal standard and off-line treatment with perchloric acid, Ara-CMP is separated from Ara-C and endogeneous matrix components on a porous graphitic carbon stationary phase using gradient elution with a water/acetonitrile mobile phase containing formic acid. The results of method performances (accuracy, linearity, limit of quantification and repeatability) show that the method is acceptable for its intended use. (c) 2006 Elsevier B.V. All rights reserved.

引用

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页码：178 / 184

页数：7

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